Preservation of goat preantral follicles in saline or coconut water solution
نویسندگان
چکیده
منابع مشابه
In vitro culture of goat preantral follicles
Background: The majority of thousands of oocytes in the ovaries are small, non-growing and reside in preantral follicles (PFs). The development of a culture system for preantral follicle may be very useful for understanding the complex mechanism in folliculogenesis at early stages of development as wells as could offer a significant way for the propagation of livestock, including goats. This pa...
متن کاملPhytohemagglutinin improves the development and ultrastructure of in vitro-cultured goat (Capra hircus) preantral follicles
The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles cultured in vitro. Secondary follicles (∼0.2 mm) were isolated from the cortex of caprine ovaries and cultured individually for 6 days in α-MEM+ supplemented with PHA (0, 1, 10, 50, 100, or 200 µg/mL). After 6 days of culture, follicle diameter ...
متن کاملProgesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro1
Lima-Verde I.B., Matos M.H.T., Celestino J.J.H., Rossetto R., Name K.P.O., Báo S.N., Campello C.C. & Figueiredo J.R. 2012. Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro. Pesquisa Veterinária Brasileira 32(4):361-367. Programa de Pós-Graduação em Ciências Veterinárias, Laboratório de Manipulação de Oócitos e Folículo...
متن کاملIn vitro culture of bovine preantral follicles.
Bovine preantral follicles (40-100 microm diameter at collection) were collected from ovaries of slaughtered cows and cultured in vitro with one of the four treatments: follicle stimulating hormone (FSH; 100 ng/ml) alone; FSH plus epidermal growth factor (EGF; 100 ng/ml); FSH plus insulin-transferrin-selenium (ITS; +1%) or FSH plus hypoxanthine (4 mM) in tissue culture medium (TCM 199) suppleme...
متن کاملEffects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles.
The maintenance of follicle quality during the transportation of ovaries is essential for the successful cryopreservation and in vitro development of preantral follicles. The objective of this study was to evaluate the effect of cooling ovarian tissue on the conservation of zebu cow preantral follicles. Ovarian pieces were immersed in saline or coconut water (CW) solutions and maintained at 4 o...
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ژورنال
عنوان ژورنال: Brazilian Journal of Veterinary Research and Animal Science
سال: 2002
ISSN: 1413-9596
DOI: 10.1590/s1413-95962002000600009